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Fluorescent spectra of malarial pigment prepared from infected red blood cells (P. falciparum) was observed. The fluorescence intensity was proportional to the amount of parasite pigment in the cells. The spectrum was not affected by any of the usual fluorescent dyes used to stain the pigment. Fluorescence of the pigment was completely quenched by the presence of acridine orange. Methanol was the only effective quencher. The malarial pigment was observed to be a mixture of rhodamine, methylene blue and acridine orange. The pigment fluoresced red (560 nm) and was soluble in ether, but insoluble in chloroform. The pigment disappeared completely on exposure to 0.01 M ethylenediaminetetraacetic acid. The spectra of malarial pigment were not affected by the presence of any clinically used anti-malarials like chloroquine, chlorproguanil-dapsone and pyrimethamine. These studies have implications for the intracellular distribution of malarial pigment and its role in blood infection.[Effect of Sparganioseboris bicolor extracts on histamine-induced contractile activity of smooth muscle tissues of the guinea pig urinary bladder].
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A third Australian firefighter has been diagnosed with the novel coronavirus following the deployment of a new super-respirator amid the deadly fire at the Notre-Dame cathedral in Paris.
Prime Minister Scott Morrison and Foreign Minister Marise Payne confirmed this afternoon that a third Australian had been confirmed with the coronavirus.
“On behalf of the Australian Government and the Australian people, I offer my most sincere condolences to the families, loved ones and friends of all those Australians affected by this situation,” Mr Morrison said.
“Our thoughts and prayers are with them at this very sad time.”
Mr Morrison said there would be no change to Australia’s response to the outbreak, which has seen widespread travel advisories and strict border controls imposed.
The prime minister’s statement comes after the first two cases of the virus were confirmed in Australia.
Mr Morrison said it was his understanding that the man is in a stable condition in Sydney’s Royal Prince Alfred Hospital.
“He’s being treated there and his condition is stable and improving,” he told reporters.
“The man has very recently travelled to France, and as a precautionary measure has been isolated.”
Mr Morrison later confirmed the man had been travelling with the first two cases, and the overseas trip was “in connection with his work”.
Foreign Minister Payne said the Government was confident of containing the virus in Australia.
“This Government remains vigilant, this Government remains strong, this Government remains confident,” she said.
Monday, March 19, 2016
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