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. 593 (2) 593 In this law… 593. 1)Exemption from income tax on salaries. 3.  2)Definition of company .Mass resonance spectroscopy: a sensitive and specific assay for platelet-activating factor.
Mass resonance spectroscopy (MRS) for platelet-activating factor (PAF, 1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine) has been developed by using our recently described source-selection algorithm for gas chromatography-mass spectrometry. MRS detection of PAF is based on the formation of ion-molecule complexes between the precursor ions and the analyte. The complexes exhibit mass shifts by a predictable amount as a function of the alkyl chain length. Since the precursor ions are selected based on their proton affinities and abilities to undergo relatively rapid and efficient gas-phase fragmentation, the molecular ions can be readily and specifically identified. MRS of PAF exhibits a unique mass spectrum, which is much different from that of PAF-like platelet-activating factor acetylhydrolase (PAF-AH). A 20-fold molar excess of nonspecific phospholipase C (PLA2) or phospholipase A2 from the venom of Agkistrodon piscivorus was unable to alter the MRS profile of either PAF-AH or PAF. Lipids bearing three or more double bonds (docosatetraenoate, stearate, palmitate, and oleate) are substrates for PAF-AH and exhibit MRS profiles similar to those of PAF. At a low subnanomolar concentration, the synthetic PAF analog, 2-acetyl-1-(O-methyl)-sn-glycero-3-phosphocholine (2-O-MePAF), is an inhibitor of PAF-AH. At a 10 micromolar concentration, 2-O-MePAF eliminated PAF from bovine serum. However, at 1 micromolar concentration, 2-O-MePAF was unable to completely inhibit PAF-AH. While the presence of a phosphate group is not a prerequisite for activation of PAF-AH, the mass spectrum of PAF is altered in the absence of a phosphate group. The presence of PAF-AH is also not a
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