Histopathologic Techniques Gregorios Pdf 26
for cells of regenerative capacity, attachment is based on [ 123 123 ] (a) inadequate nutrition of undifferentiated monolayers of cells, (b) low density of cell seeding, and (c) the inability of certain cell types to migrate. as a result, monolayers may not become confluent, and cell growth is characterized by separate islands of proliferating cells which are separated by cytoplasm and many times by a layer of extracellular matrix or matrix remnants. this phenomena results in a less flat aspect and reduces the penetration capacity. a feature of less than optimal adherence is the formation of islands, which are nonattached and unjoined to other islands.
the method of choice for the detection of circulating tumor cells is immunocytochemistry. cd45 antigen is commonly used for identifying haematopoietic cells in cytology specimens. the other distinguishing feature of staining for the detection of ctcs is the need for the generation of a novel culture system that improves the recovery of the tumor cells.
histopathologic, cytogenetic, and electron microscopic studies have revealed the polyploidization of the replicating chromosomes in chinese hamster ovary (cho) cells. the effects of different factors (e.g.
the organisms that have garnered the most attention are bartonella species, chlamydia species, c burnetii, brucella species, legionella species, tropheryma whipplei, candida, and non-candida fungi (particularly aspergillus species). the last 2 groups of organisms are especially relevant to pv recipients. with the use of special diagnostic techniques, bartonella species, c burnetii, and brucella species have been identified in the majority of cases of culture-negative ie caused by fastidious organisms. additional laboratory screening is required to identify the causes of culture-negative ie. 233 in some cases, serological and special blood culture techniques can be helpful. in other cases, tissue (usually valve) screening is required. diagnostic methods for resected valve tissue include microbiological, histopathological, and molecular techniques, the last of which includes gene amplification with pcr methods. unfortunately, most clinical laboratories do not perform molecular screening, and specimens must be sent to reference laboratories.