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It has been shown that genistein influences a different set of genes in the malignant breast cancer cell lines compared to those in the normal cells. Particularly, the function of p53 as a driver of apoptosis in the presence of genistein and estrogens, as well as the role of cell cycle checkpoint regulation, epigenetic alteration, metastasis, cell invasion, and angiogenesis. The above-mentioned roles in inducing apoptosis suggest a possible link between the effect of genistein and the p53 status \[[@B55-ijms-20-02913],[@B59-ijms-20-02913]\]. The influence of p53 on apoptotic and angiogenic pathways is described in a subsequent section.

As described in the present study, the use of the structural hypothesis and functional studies that consider the effect of genistein on the expression and regulation of p53, allows for the identification of targets and the better understanding of the breast cancer molecular mechanisms; and to assess the pathophysiological mechanism that might be involved in patients with this disease, as well as the identification of the role of p53 in the presence of genistein. In addition, the previous analysis of the functional role of p53 in cancerous cells (using genistein and doxorubicin) highlighted that the modification of p53-induced apoptosis is
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